Broad-spectrum enzymatic inhibition of CRISPR-Cas12a
نویسندگان
چکیده
منابع مشابه
CRISPR-Cas12a-Assisted Recombineering in Bacteria
Clustered regularly interspaced short palindromic repeat (CRISPR)-Cas12a (Cpf1) has emerged as an effective genome editing tool in many organisms. Here, we developed and optimized a CRISPR-Cas12a-assisted recombineering system to facilitate genetic manipulation in bacteria. Using this system, point mutations, deletions, insertions, and gene replacements can be easily generated on the chromosome...
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ژورنال
عنوان ژورنال: Nature Structural & Molecular Biology
سال: 2019
ISSN: 1545-9993,1545-9985
DOI: 10.1038/s41594-019-0208-z